Printer: Nanoscribe GT
Print Time: 3.5hrs
Material: IP Dip
This project is an ongoing collaboration between myself, NIH and UMD's MML and BAM labs. The ultimate goal is to repair damaged (broken) nerve bundles. We are first attempting to guide some neurons through these channels and understand how they behave as they navigate through a changing volume. I have designed these structures myself, and varied several parameters of each of these periodic channels. The channels printed had a diameter of approx 3um. After printing and developing, we molded them in PDMS. The positive is capable of being remolded given that we have achieved a well adhesion to the substrate
The writing area of the Nanoscribe GT system is relatively limited. The optimal writing block for a 63x objective is 100um^3. That being said, I have developed optimal structures that extend up to 230um^3. In order to create structures that are capable of extending to the macro scale, one must use the stitching function. The lines observed in the image are the product of stitching.